Co-Pitching Yeasts: S. cerevisiae and Brettanomyces

by John Brewster
4 minutes read
Co-Pitching Yeasts: S. cerevisiae and Brettanomyces

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Co-pitching Saccharomyces cerevisiae and Brettanomyces simultaneously is a technique for producing Brett character integrated into the fermentation from pitch rather than added as a secondary organism months later. I’ve brewed the same saison recipe with sequential Brett addition (Brett added in secondary at 4 weeks) and co-pitch (both organisms pitched together at day one), and the flavor difference is significant, co-pitch produces a fundamentally different, more integrated character than sequential inoculation.

Co-pitching dynamics: how the organisms interact

Competition and succession: When Saccharomyces cerevisiae and Brettanomyces bruxellensis are pitched simultaneously into fresh wort, Saccharomyces dominates the initial fermentation. S. cerevisiae has a faster growth rate, higher alcohol tolerance, and more efficient simple sugar metabolism, it ferments the bulk of the available glucose and maltose in the first 4–7 days of fermentation. Brettanomyces, while present from pitch, grows more slowly and represents a small percentage of the fermenting population during primary fermentation. After Saccharomyces completes primary fermentation and begins to flocculate out, the conditions shift in Brettanomyces’s favor: Brett has superior ability to ferment dextrins (long-chain carbohydrates) that S. cerevisiae cannot access, and Brett’s slower metabolism is no longer disadvantaged when the primary fermentation rush is complete. Brett takes over the long secondary fermentation, slowly fermenting residual dextrins and producing its characteristic flavor compounds (4-ethylphenol, 4-ethylguaiacol, isovaleric acid, ethyl esters). Co-pitch vs. sequential addition character differences: Sequential addition (Brett added in secondary after Saccharomyces finishes): produces a clear two-phase character, a Saccharomyces-primary beer with Brett flavor laid on top. The Brett character develops separately and can taste somewhat grafted onto the base beer rather than integrated. Co-pitch: produces a more seamless integration because both organisms’ metabolic activity overlaps from the beginning of fermentation. Saccharomyces esters and Brett esters form in parallel rather than sequentially, creating a more complex, layered character where the primary fermentation flavor and Brett character are intertwined. Ratio considerations: A typical co-pitch ratio is 80–90% Saccharomyces cells to 10–20% Brettanomyces cells by count. Higher Brett percentage accelerates Brett character development but risks undermining clean primary fermentation if Brett’s acid production stresses the Saccharomyces before primary fermentation completes. Higher Saccharomyces percentage produces a cleaner primary fermentation with Brett developing more slowly in the later stages, closer in character to a sequential addition but with earlier Brett establishment.

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Style applications and pitching recommendations

Best styles for co-pitching: Belgian saison with Brett character, one of the most classic applications, producing the complex saison-Brett hybrid character associated with the Orval style. Pitch a saison strain (Wyeast 3724, Belle Saison, or T-58) with Brettanomyces bruxellensis (White Labs WLP650 or Omega Brett B) at 85:15 ratio by volume of commercial packages. Ferment at 22–24°C for full saison character development alongside Brett establishment. Wild farmhouse ales and Bière de Garde with Brett influence. American Wild Ales where controlled Brett character is a design element rather than an uncontrolled variable. Brettanomyces strain selection for co-pitch: Brett bruxellensis produces the classic leather-barnyard-spice character (4-ethylphenol dominant). Brett anomalus produces more fruity-tropical Brett ester character (3-methyl-1-butanol, ethyl caproate). Brett clausenii produces the mildest Brett character, subtle, slightly fruity. For co-pitch with pronounced farmhouse character target: Brett bruxellensis. For co-pitch where Brett should integrate subtly as a fruity complexity layer: Brett clausenii or anomalus. Timeline: Co-pitch beers require extended conditioning: minimum 3–4 months at fermentation temperature (20–22°C) before packaging for the Brett character to fully develop. Packaging before Brett fermentation completes risks over-carbonation as Brett continues to ferment in bottle. Measure gravity at packaging, if stable at the same reading over 2 consecutive weeks and Brett character is at the desired level, the beer is ready to package at low carbonation.

Common Questions

Will co-pitched Brett contaminate my other equipment permanently?

Brettanomyces contamination risk is real and the concern about equipment contamination is legitimate, Brett is notoriously difficult to fully eliminate from porous surfaces and plastic equipment once established. The contamination risk from co-pitch fermentation depends entirely on equipment type and sanitation protocol. Plastic fermenters, plastic tubing, and wooden equipment: Brett can colonize micro-scratches and pores in plastic surfaces and is extremely difficult to eliminate with standard sanitizers. Equipment used for Brett fermentation should be dedicated exclusively to Brett beers, never use Brett-contaminated plastic equipment for clean beer production. Glass and stainless steel: Brett cannot colonize smooth, non-porous surfaces if properly sanitized. A glass carboy or stainless fermenter used for a Brett beer, cleaned with hot caustic (PBW at 60°C) and sanitized with Star San, is effectively decontaminated for subsequent use. Peracetic acid (PAA) is more effective than Star San against Brett in commercial settings. Practical recommendation: use a dedicated plastic or cheaper fermenter for Brett beers, and reserve clean plastic equipment for Saccharomyces-only production. Alternatively, invest in a stainless fermenter for Brett beers, the non-porous surface makes decontamination reliable. Hoses, racking canes, and auto-siphons that contact Brett wort should be replaced rather than sanitized for clean beer use, as Brett can persist in hose connectors and micro-pores despite sanitization. The contamination risk is a real management consideration but not a reason to avoid Brett brewing, it just requires equipment segregation planning before starting.

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